LOCUS       sequence-133444-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Salmon P
  TITLE     Generation of human cell lines using lentiviral-mediated genetic 
            engineering.
  JOURNAL   Methods Mol Biol. 2013;945:417-48. doi: 
            10.1007/978-1-62703-125-7_25.
  PUBMED    23097121
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2013"; volume: "945:417-48. doi"; pages: " 
            10.1007/978-1-62703-125-7_25"
COMMENT     Sequence Label: pCLX-UBI-GFP
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(160..162,163..165,166..879)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(160..162,163..165,166..876)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(205..226)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(466..485)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     813..834
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(880..904)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(975..995)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
       61 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
      121 ggatcccaac tttgtacaaa aaagcaggct cccgccacca tggtgagcaa gggcgaggag
      181 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      241 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      301 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      361 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      421 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      481 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      541 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      601 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      661 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      721 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      781 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      841 gccgggatca ctctcggcat ggacgagctg tacaagtaag acccagtttc ttgtacaaag
      901 tggtgataat cgaattccga taatcaacct ctggattaca aaatttgtga aagattgact
      961 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     1021 tatcatgcta ttgcttcccg
//
