LOCUS       sequence-133447-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression vector with pBabe compatible MCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  TITLE     Sherr/Roussel lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSCV-pBabeMCS-IRES-RFP
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    99..648
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(243..260)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     470..489
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttgggatccc agtgtggtgg tacgtaggaa
       61 ttcagatctc gagcgggatc aattccgccc cccccctaac gttactggcc gaagccgctt
      121 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
      181 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
      241 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
      301 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
      361 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
      421 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
      481 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
      541 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
      601 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc atgaccatgg
      661 cctcctccga ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg
      721 tgaacggcca cgagttc
//
