LOCUS       sequence-133450-         490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, WPRE, and bGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
  PUBMED    24618276
REFERENCE   2  (bases 1 to 490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2014-03-11"; volume: "7:17. doi"; pages: " 
            10.1186/1756-6606-7-17"
COMMENT     Sequence Label: pAAV-CWB-EGFP
FEATURES             Location/Qualifiers
     source          1..490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggctagc tcgaatcaag cttatcgata ccgtcttttc
       61 acgctgcccc cagaactagg ggccactcgc ctgcccgtgc tcctgagtgc aaacggagaa
      121 ccggcttgac tgacgagctt ggggcttctg agcagggcac tgtggctgct cacagcctcg
      181 cgagccttcg tcagcatcca ggtccccatg gcaaccacct ccgaaacgcc ctgttcccgt
      241 tgccccggta actgcctccc caacacctgc ctgcctttca ctttaaaacc tgctcctctt
      301 tgccctggcc ttatatacac aatactgcag gactgtgtgg gcccaggagc aagtggaccc
      361 tgttccccca gggtgatgta ctgagggggt tggagaaggg gatgcgaaca aacttagtcc
      421 acaagtgtgc agcatctgcg gccgcaggaa cccctagtga tggagttggc cactccctct
      481 ctgcgcgctc
//
