LOCUS       sequence-133461-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN/ARS yeast shuttle vector with DS2 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Rugbjerg P, Myling-Petersen N, Sommer MO
  TITLE     Flexible metabolic pathway construction using modular and divisible 
            selection gene regulators.
  JOURNAL   Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. 
            Epub 2015 Aug 21.
  PUBMED    26303342
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:189-97. doi"; pages: " 
            10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21"
COMMENT     Sequence Label: pDS2
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(400..433)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgat ttgcccgggc
      301 agttcagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      361 attgggtgca taggccacta gtggatctga tatcacctaa taacttcgta tagcatacat
      421 tatacgaagt tatattaagg gttctcgaga gctcgttttc gacactggat ggcggcgtta
      481 gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac
      541 tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat
      601 cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa
      661 gttcttgaaa acaagaatct ttttattgtc ctcgagttac cgcttctaga gcagcagaca
      721 tgatttcttt ttaggcttct tcttttccac tggcgttttc ctatttatct gtctgaccag
      781 gtcataaaat atctcattaa cattgatctt tgactttgca ga
//
