LOCUS       sequence-133462-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN/ARS yeast shuttle vector with DS2 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Rugbjerg P, Myling-Petersen N, Sommer MO
  TITLE     Flexible metabolic pathway construction using modular and divisible 
            selection gene regulators.
  JOURNAL   Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. 
            Epub 2015 Aug 21.
  PUBMED    26303342
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:189-97. doi"; pages: " 
            10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21"
COMMENT     Sequence Label: pDS2
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..22)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    64..567
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        604..708
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg
       61 acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg
      121 gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga aagtaaataa
      181 agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta aaaaatttca
      241 acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta aatagatata
      301 cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca
      361 cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa gataaaaggt
      421 agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa aactattttt
      481 tctttaattt ctttttttac tttctatttt taatttatat atttatatta aaaaatttaa
      541 attataatta tttttatagc acgtgatgaa aaggacccag gtggcacttt tcggggaaat
      601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgg
//
