LOCUS       sequence-133469-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9-HF2 variant: 
            CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, 
            D1135E)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2135
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctggcaaac ggagagatac gcaaacgacc tttaattgaa accaatgggg agacaggtga
       61 aatcgtatgg gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca
      121 agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct
      181 tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta
      241 cggtggcttc gagagcccta cagttgccta ttctgtccta gtagtggcaa aagttgagaa
      301 gggaaaatcc aagaaactga agtcagtcaa agaattattg gggataacga ttatggagcg
      361 ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa
      421 aaaggatctc ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa
      481 acggatgttg gctagcgccg gagagcttca aaaggggaac gaactcgcac taccgtctaa
      541 atacgtgaat ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga
      601 taacgaacag aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga
      661 gcaaatttcg gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt
      721 aagcgcatac aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca
      781 tttgtttact cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat
      841 agatcgcaaa cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc
      901 catcacggga
//
