LOCUS       sequence-133471-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9-HF4 variant: 
            CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, 
            Q926A)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2133
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agatacgcaa acgaccttta attgaaacca atggggagac aggtgaaatc gtatgggata
       61 agggccggga cttcgcgacg gtgagaaaag ttttgtccat gccccaagtc aacatagtaa
      121 agaaaactga ggtgcagacc ggagggtttt caaaggaatc gattcttcca aaaaggaata
      181 gtgataagct catcgctcgt aaaaaggact gggacccgaa aaagtacggt ggcttcgata
      241 gccctacagt tgcctattct gtcctagtag tggcaaaagt tgagaaggga aaatccaaga
      301 aactgaagtc agtcaaagaa ttattgggga taacgattat ggagcgctcg tcttttgaaa
      361 agaaccccat cgacttcctt gaggcgaaag gttacaagga agtaaaaaag gatctcataa
      421 ttaaactacc aaagtatagt ctgtttgagt tagaaaatgg ccgaaaacgg atgttggcta
      481 gcgccggaga gcttcaaaag gggaacgaac tcgcactacc gtctaaatac gtgaatttcc
      541 tgtatttagc gtcccattac gagaagttga aaggttcacc tgaagataac gaacagaagc
      601 aactttttgt tgagcagcac aaacattatc tcgacgaaat catagagcaa atttcggaat
      661 tcagtaagag agtcatccta gctgatgcca atctggacaa agtattaagc gcatacaaca
      721 agcacaggga taaacccata cgtgagcagg cggaaaatat tatccatttg tttactctta
      781 ccaacctcgg cgctccagcc gcattcaagt attttgacac aacgatagat cgcaaacgat
      841 acacttctac caaggaggtg ctagacgcga cactgattca ccaatccatc acgggattat
      901 atgaaactcg gatagatttg tcacagcttg ggggtgacgg atcccccaga
//
