LOCUS       sequence-133472-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9-VQR-HF1 variant: 
            CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, 
            R1335Q, T1337R)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2440
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..95)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(102..122)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggtgatga tgaccggtca tcctgcagcc ttgtcatcgt catccttgta atcgatgtca
       61 tgatctttat aatcaccgtc atggtctttg tagtcgctcg agactttcct cttcttcttg
      121 ggggatccgt cacccccaag ctgtgacaaa tctatccgag tttcatataa tcccgtgatg
      181 gattggtgaa tcagtgtcgc gtctagcacc tccttggtag atctgtactg tttgcgatct
      241 atcgttgtgt caaaatactt gaatgcggct ggagcgccga ggttggtaag agtaaacaaa
      301 tggataatat tttccgcctg ctcacgtatg ggtttatccc tgtgcttgtt gtatgcgctt
      361 aatactttgt ccagattggc atcagctagg atgactctct tactgaattc cgaaatttgc
      421 tctatgattt cgtcgagata atgtttgtgc tgctcaacaa aaagttgctt ctgttcgtta
      481 tcttcaggtg aacctttcaa cttctcgtaa tgggacgcta aatacaggaa attcacgtat
      541 ttagacggta gtgcgagttc gttccccttt tgaagctctc cggcgctagc caacatccgt
      601 tttcggccat tttctaactc aaacagacta tactttggta gtttaattat gagatccttt
      661 tttacttcct tgtaaccttt cgcctcaagg aagtcgatgg ggttcttttc aaaagacgag
      721 cgctccataa tcgttatccc caataattct ttgactgact tcagtttctt ggattttccc
      781 ttctcaactt ttgccactac taggacagaa taggcaactg tagggctcac gaagccaccg
      841 tactttttcg ggtcccagtc c
//
