LOCUS       sequence-133478-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9-VRQR-HF1 variant: 
            CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, 
            G1218R, R1335Q, T1337R)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2443
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agacggaaat cactctggca aacggagaga tacgcaaacg acctttaatt gaaaccaatg
       61 gggagacagg tgaaatcgta tgggataagg gccgggactt cgcgacggtg agaaaagttt
      121 tgtccatgcc ccaagtcaac atagtaaaga aaactgaggt gcagaccgga gggttttcaa
      181 aggaatcgat tcttccaaaa aggaatagtg ataagctcat cgctcgtaaa aaggactggg
      241 acccgaaaaa gtacggtggc ttcgtgagcc ctacagttgc ctattctgtc ctagtagtgg
      301 caaaagttga gaagggaaaa tccaagaaac tgaagtcagt caaagaatta ttggggataa
      361 cgattatgga gcgctcgtct tttgaaaaga accccatcga cttccttgag gcgaaaggtt
      421 acaaggaagt aaaaaaggat ctcataatta aactaccaaa gtatagtctg tttgagttag
      481 aaaatggccg aaaacggatg ttggctagcg ccagagagct tcaaaagggg aacgaactcg
      541 cactaccgtc taaatacgtg aatttcctgt atttagcgtc ccattacgag aagttgaaag
      601 gttcacctga agataacgaa cagaagcaac tttttgttga gcagcacaaa cattatctcg
      661 acgaaatcat agagcaaatt tcggaattca gtaagagagt catcctagct gatgccaatc
      721 tggacaaagt attaagcgca tacaacaagc acagggataa acccatacgt gagcaggcgg
      781 aaaatattat ccatttgttt actcttacca acctcggcgc tccagccgca ttcaagtatt
      841 ttgacacaac gatagatcgc aaacagtaca gatctaccaa ggaggtgcta gacgcgacac
      901 tgattcacca atccatcacg ggattatatg aaactcggat agatttgtca cagcttgggg
      961 g
//
