LOCUS       sequence-133488-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Young RL, Korsmeyer SJ
  TITLE     A negative regulatory element in the bcl-2 5'-untranslated region 
            inhibits expression from an upstream promoter.
  JOURNAL   Mol Cell Biol 1993 Jun;13(6):3686-97.
  PUBMED    8388542
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1993-06"; volume: "13(6)"; pages: "3686-97"
COMMENT     Sequence Label: 2674 pBS Bcl-2 P1+P2
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    105..226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(116..135)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     170..189
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(651..671)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          801..866
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 atcccccggg ctgcaggaat tcgatatcaa gcttgcagac atgataagat acattgatga
      121 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      181 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      241 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      301 cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat
      361 tccttattaa cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta
      421 ttaatagcag acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa
      481 ctgttatgcc tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt
      541 gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca
      601 gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc
      661 ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc
      721 atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca
      781 tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat
      841 tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
      901 aattatgtca caccacagaa gtaaggttcc ttc
//
