LOCUS       sequence-133489-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast chr. integration vector with DS3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Rugbjerg P, Myling-Petersen N, Sommer MO
  TITLE     Flexible metabolic pathway construction using modular and divisible 
            selection gene regulators.
  JOURNAL   Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. 
            Epub 2015 Aug 21.
  PUBMED    26303342
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:189-97. doi"; pages: " 
            10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21"
COMMENT     Sequence Label: pDS3-X4
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(61..94)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(678..702)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atacgactca ctatagggcg aattgggtgc ataggccact agtggatctg atatcaccta
       61 ataacttcgt atagcataca ttatacgaag ttatattaag ggttctcgag agctcgtttt
      121 cgacactgga tggcggcgtt agtatcgaat cgacagcagt atagcgacca gcattcacat
      181 acgattgacg catgatatta ctttctgcgc acttaacttc gcatctgggc agatgatgtc
      241 gaggcgaaaa aaaatataaa tcacgctaac atttgattaa aatagaacaa ctacaatata
      301 aaaaaactat acaaatgaca agttcttgaa aacaagaatc tttttattgt cctcgagtta
      361 ccgcttcttt aggatgaagt catagttggc ggcagagttc atggcataga acacattggc
      421 gttctctgga attttcagct tgtggtcctc tgagatgatc tgcagcagct catagtcctc
      481 gggctcgtcc tcatctaggt tgtgtttgtc catggctttc cggatgacag tcggagcctt
      541 atcctggctg gtcaccagga tgctcttgta catgttgccg ttgtccacat ccaggctgac
      601 cctgatgatg cagcagtcgc ccacctgctg gttgtagagt ggcagtgagg agctggaggt
      661 gaagggggcg gccgcagagc ctgctttttt gtacaaactt gtttgattcg acccaccgta
      721 ctcgtcaatt ccaagggcat cggtaaacat ctgctcaaac tcgaagtcgg ccatatccag
      781 agcgccgtag gg
//
