LOCUS       sequence-133490-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast chr. integration vector with DS3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Rugbjerg P, Myling-Petersen N, Sommer MO
  TITLE     Flexible metabolic pathway construction using modular and divisible 
            selection gene regulators.
  JOURNAL   Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. 
            Epub 2015 Aug 21.
  PUBMED    26303342
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:189-97. doi"; pages: " 
            10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21"
COMMENT     Sequence Label: pDS3-X4
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(21..427)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     protein_bind    666..699
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cctgcacttt gtaattaaaa cttagattag attgctatgc tttctttcta atgagcaaga
       61 agtaaaaaaa gttgtaatag aacaagaaaa atgaaactga aacttgagaa attgaagacc
      121 gtttattaac ttaaatatca atgggaggtc atcgaaagag aaaaaaatca aaaaaaaaaa
      181 ttttcaagaa aaagaaacgt gataaaaatt tttattgcct ttttcgacga agaaaaagaa
      241 acgaggcggt ctcttttttc ttttccaaac ctttagtacg ggtaattaac gacaccctag
      301 aggaagaaag aggggaaatt tagtatgctg tgcttgggtg ttttgaagtg gtacggcgat
      361 gcgcggagtc cgagaaaatc tggaagagta aaaaaggagt agaaacattt tgaagctatg
      421 gtgtgtgcat cgcgtgcatt catccgctct aaccgaaaag gaaggagtta gacaacctga
      481 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
      541 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
      601 cttgcttgag aaggttttgg gacgctccga agatcgcgtc ataacagctt tcgacaaccc
      661 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctagag atctgtttag
      721 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
      781 gacagtcttg acgtgcgcag ctcagg
//
