LOCUS       sequence-133501-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..80
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             84..104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             876..929
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcggcgcgcc agtcctccga cagactgagt cgcccgctcg agccaccatg ggatatccat
       61 acgatgtccc agattatgcg ccacctaaga agaaacgcaa agtccccggg gatcttacgt
      121 acgcgtattt agttggtctc tacgaagggg atggatactt tagtatcacc aagaaaggca
      181 agtacttgac ttatgaatta ggtattgagc tgagcatcaa agacgtccaa ttgatttaca
      241 agatcaagaa aatcctaggt attggcatcg taagcttcag gaagagaaac gagattgaaa
      301 tggttgcatt gaggatccgt gataagaatc atctaaaatc taagatattg cctatatttg
      361 agaagtatcc aatgttttcc aacaaacagt acgactattt aagattcagg aatgcattgt
      421 tatctggcat tatataccta gaagacttgc ctgattacac tagaagtgac gaaccattga
      481 attctataga atccattatc aacacatcat acttctccgc ctggctagtt ggatttatag
      541 aagctgaggg ctgtttcagt gtgtacaagc tgaacaaaga cgatgactac ttgattgctt
      601 cattcgacat tgcccaaaga gatggtgata tcttgatttc agcaattagg aagtacttaa
      661 gtttcactac taaggtttac ctagacaaga ctaattgtag caaattgaag gtcactagtg
      721 ttagatccgt cgagaacatc attaagtttc tgcagaatgc tcctgtcaaa ttgttaggca
      781 acatgaaact gcaatacaag ttgtggttga aacaactaag gaagatttct aggtattccg
      841 agaagatcaa gattccatca aactacggat ccggtgaggg cagaggaagt cttctaacat
      901 gcggtgacgt ggaggagaat ccgggcccca tgagcgagct gattaaggag aacatgcaca
      961 tga
//
