LOCUS       sequence-133511-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Young JA, Sermwittayawong D, Kim HJ, Nandu S, An N, 
            Erdjument-Bromage H, Tempst P, Coscoy L, Winoto A
  TITLE     Fas-associated death domain (FADD) and the E3 ubiquitin-protein 
            ligase TRIM21 interact to negatively regulate virus-induced 
            interferon production.
  JOURNAL   J Biol Chem. 2011 Feb 25;286(8):6521-31. Epub 2010 Dec 23.
  PUBMED    21183682
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-02-25"; volume: "286(8)"; pages: "6521-31. Epub 
            2010 Dec 23"
COMMENT     Sequence Label: pCI-His-hUbi
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          145..277
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     260..279
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        322..340
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             349..366
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             367..591
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             367..591
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    640..761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(651..670)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     705..724
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
       61 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga
      121 agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac
      181 caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt
      241 ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac
      301 agctcttaag gctagagtac ttaatacgac tcactatagg ctagcatgca ccaccatcac
      361 catcatcaga tcttcgtgaa gaccctgact ggtaagacca tcactctcga agtggagccg
      421 agtgacacca ttgagaatgt caaggcaaag atccaagaca aggaaggcat ccctcctgac
      481 cagcagaggt tgatctttgc tgggaaacag ctggaagatg gacgcaccct gtctgactac
      541 aacatccaga aagagtccac cctgcacctg gtactccgtc tcagaggtgg gtgaggtacc
      601 tctagagtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
      661 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      721 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      781 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
      841 acaaatgtgg taaaatcgat aa
//
