LOCUS       sequence-133512-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TetActivator protein under the control of the c-fos 
            promoter for use in TetTaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia 
            AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding 
            EC, Wisden W, Franks NP
  TITLE     Neuronal ensembles sufficient for recovery sleep and the sedative 
            actions of alpha2 adrenergic agonists.
  JOURNAL   Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015
            Feb 23.
  PUBMED    25706476
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3957"; 
            journalName: "Nat Neurosci"; date: "2015-04"; volume: "18"; issue: 
            "4"; pages: "553-61"
COMMENT     Sequence Label: pAAV-cFos-tTA-pA
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(833..886)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gaatcgatgc ggccagacac tggtgggagc tgcagagcag agctgggtgg gagcgcggtc
       61 actgctcgtt cgcggaaccg ccggctctat ccagtcttct cagttgctcg ctgcagtcgc
      121 ggttggagta gtaggcgcct cagctggcgc ctttatagaa gcgctgtgaa tggatggact
      181 tcctacgtca ctgggcggaa ctggaagggg ggggaagcgc gccccaaggc cctctgcggg
      241 cgctctgtcg tcaactctac gccccaggct ttcaaccccc gtcttggcat acatctttca
      301 cctatgagtg ttcacatttg ggatcttagg gggtctccta gaccttccgc gtgtaggatt
      361 tcggagatgg tccccccccc agaacaacag ggaccggccg tggaaacctg ctgacgcaga
      421 tgtcctaata tggacatcct gtgtaaagga gggagggatt gacgggaaca gctcgccggc
      481 tgcagccaac tctgagggtg cagcgcaggg ggggaaccgg ggccgcggcc gggggagggg
      541 aggcgggaaa ggcagagaag gcgagcgaac attcgcacct gattcaatgt ggacccggtt
      601 cccagggtca agggatgaga cccgggtcat tgtccagcaa tctggaactt tttttttttt
      661 tttgagtctc tggtattagg agaagcaagt acgcagcctt aaaggctcga agcctctgtt
      721 cctaaaggaa agctgcggcc gtctggctta attaaccgtc cagctcgacc aggatgggca
      781 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggatcccggt gcagggccgg
      841 gattctcctc cacgtcaccg catgttagaa gacttcctct gccctcgagg tagataagta
      901 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg cca
//
