LOCUS       sequence-133527-       11679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Wong AS, Choi GC, Cheng AA, Purcell O, Lu TK
  TITLE     Massively parallel high-order combinatorial genetics in human cells.
  JOURNAL   Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 
            2015 Aug 17.
  PUBMED    26280411
REFERENCE   2  (bases 1 to 11679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3326"; 
            journalName: "Nat Biotechnol"; date: "2015-09"; volume: "33"; issue:
            "9"; pages: "952-61"
COMMENT     Sequence Label: pAWp7-12
FEATURES             Location/Qualifiers
     source          1..11679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..608
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(73..93)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(491..502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(611..627)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(612..628)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1133..1313
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1339..1356)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1345..1569
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1615..2043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1702..1721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1912..1933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2052..2072)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2057..2386
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2237..2372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2299..2318
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2434..2481
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2500..2874
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3004..3125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3041..3060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3095..3114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3174..3190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3174..3190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3187..3209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3198..3214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3222..3228,3229..3246,3247..3252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3267..3288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3405..3422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3576..4164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3656..3675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4335..5126,5127..5195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4958..4977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5196..5300)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5375..5394)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5566..5945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5947..6145
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6163..6343
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6390..6515
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7008..7241
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7426..7470
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7768..7885
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7939..9150
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8987..9006
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             9167..9862
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     enhancer        9991..10370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10371..10574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      10593..10602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10599..10601,10602..10604,10605..11318)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10599..10601,10602..10604,10605..11315)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10644..10665)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10905..10924)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11252..11273
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
       61 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
      121 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
      181 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
      241 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
      301 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
      361 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
      421 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
      481 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
      541 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
      601 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
      661 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
      721 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
      781 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
      841 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
      901 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
      961 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     1021 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     1081 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     1141 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     1201 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     1261 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     1321 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     1381 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     1441 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     1501 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     1561 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     1621 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     1681 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     1741 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     1801 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     1861 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     1921 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     1981 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2041 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     2101 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2161 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2221 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2281 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     2341 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     2401 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     2461 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     2521 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     2581 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     2641 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     2701 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     2761 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     2821 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     2881 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     2941 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     3001 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3121 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     3181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     5341 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5401 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     5461 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     5521 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     5581 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5641 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     5701 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     5761 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     5821 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     5881 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     5941 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     6001 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     6061 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     6121 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     6181 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6241 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6301 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     6361 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     6421 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     6481 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     6541 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     6601 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     6661 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     6721 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     6781 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     6841 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     6901 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     6961 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     7021 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     7081 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     7141 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     7201 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     7261 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     7321 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     7381 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     7441 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     7501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     7561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     7621 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     7681 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     7741 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     7801 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     7861 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     7921 gttaattaag ggtgcagcgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     7981 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     8041 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     8101 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     8161 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     8221 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     8281 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     8341 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     8401 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     8461 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     8521 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     8581 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     8641 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     8701 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     8761 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     8821 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     8881 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     8941 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     9001 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     9061 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     9121 ttctggccgt ttttggcttt tttgttagac agttaattaa gccaccatga gcgagctgat
     9181 caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     9241 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt
     9301 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     9361 cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     9421 tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac
     9481 ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa
     9541 cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga
     9601 gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt
     9661 gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa
     9721 gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc
     9781 cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc
     9841 tagcaaactg gggcacagat aataacctgc aaaaacgacc atggctgtag actgttacga
     9901 ccatggctgt agactgttac gaccatggct gtagactgtt acgaccatgg ctgtagactg
     9961 ttaaatgcag gcgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
    10021 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    10081 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    10141 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    10201 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    10261 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    10321 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    10381 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    10441 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    10501 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    10561 atataagcag cggtaacccc cgggtaccgg tcgccaccat ggtgagcaag ggcgaggagc
    10621 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
    10681 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
    10741 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
    10801 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
    10861 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
    10921 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
    10981 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
    11041 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
    11101 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
    11161 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
    11221 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
    11281 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagagatc
    11341 tagagaggag gatccgcgaa aagtccggaa ttcaggtggg agtactgctc agagctacaa
    11401 ctctaggagt agggactcca agcctagagc tccaagagag ggtgaagggc agggagaaaa
    11461 ttatagtaat agttgcaatg agtcacttgc ttctagatca agatcagaga ctctgctctc
    11521 cgtgttcaca gcggaccttg atttaatgtc atacaattaa ggcacgcggt gaatgccaag
    11581 agcggagcct acagctgcac ttgaaggaca tccgagagaa gttaggaagg gtggggagaa
    11641 acaattctag aatgaaccca tcctgtgcga caccaattg
//
