LOCUS       sequence-133620-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..672
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagtgtg attaaaccag agatgaagat gaggtactac
       61 atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac aggcagacct
      121 tacgagggac atcaagagat gacactacgc gtcacaatgg ccgagggcgg gccaatgcct
      181 ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagagtatt tactaaatat
      241 ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg
      301 tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag ccttagagga
      361 aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga tggtcctatc
      421 atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc cagcgacgga
      481 gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc
      541 caaatgaaga ctacttacaa ggcggcaaaa gagattcttg aaatgccagg agaccattac
      601 atcggccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagcaggt agaagatgca
      661 gtagctcata gctccggact cagatctcga gccatgacct ctaggaagaa agtgttgctg
      721 aaggttatca tcctgggaga tt
//
