LOCUS       sequence-133627-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette upstream of the
            firefly luciferase gene and the DSCP core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pGL3-Gateway-DSCP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..131
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(156..161,162..179,180..186)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(306..325)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtaccacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
       61 atattaaatt agattttgca taaaaaacag actacataat actgtgaaac acaacatatc
      121 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
      181 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
      241 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      301 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      361 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      421 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
      481 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      541 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      601 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      661 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      721 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      781 acaaggtgct gatgccgctg gcgattcagg tt
//
