LOCUS       sequence-133630-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette downstream of 
            the firefly luciferase gene and the DSCP core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pGL3-DSCP-Gateway
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..136
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(177..482)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(279..298)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agcggaagag caccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata
       61 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      121 atatccagtc actatggtcg acctgcagac tggctgtgta taagggagcc tgacatttat
      181 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
      241 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
      301 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc
      361 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc
      421 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
      481 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
      541 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
      601 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
      661 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
      721 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
      781 acgcatactg ttatctggct tt
//
