LOCUS       sequence-133631-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  humanSTARR-seq validation vector with Gateway cassette upstream of 
            the firefly luciferase gene and the SCP1 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pGL4-Gateway-SCP1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..138
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(163..168,169..186,187..193)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(313..332)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 actggccggt accacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
       61 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtgaaacaca
      121 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
      181 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      241 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      301 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      361 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      421 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      481 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      541 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      601 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      661 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
      721 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
      781 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatgg
//
