LOCUS       sequence-133634-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette downstream of 
            the firefly luciferase gene and the RpS12 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pGL3-RpS12-Gateway
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(151..456)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(253..272)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
       61 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc
      121 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
      181 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
      241 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
      301 cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac
      361 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
      421 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag
      481 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
      541 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac
      601 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
      661 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
      721 tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt
      781 aagccggatc cacgcgttta cgcc
//
