LOCUS       sequence-133636-        1242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The retroviral GOT1 vector was generated by cloning an sgRNA 
            resistant human GOT1 gene block into the pMXS-ires-blast vector by 
            Gibson Assembly..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
  TITLE     An Essential Role of the Mitochondrial Electron Transport Chain in 
            Cell Proliferation Is to Enable Aspartate Synthesis.
  JOURNAL   Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.
  PUBMED    26232224
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "540-51"
COMMENT     Sequence Label: PMXS-GOT1
FEATURES             Location/Qualifiers
     source          1..1242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcacctc cgtcagtctt tgccgaggtt ccgcaggccc agcctgtcct ggtcttcaag
       61 ctcactgccg acttcaggga ggatccggac ccccgcaagg tcaacctggg agtgggagca
      121 tatcgcacgg atgactgtca cccatgggtt ttgccagtag tgaagaaagt ggagcagaag
      181 attgctaatg acaatagcct aaatcacgag tatctgccaa tcctgggcct ggctgagttc
      241 cggagctgtg cttctcgtct tgcccttggg gatgacagcc cagcactcaa ggagaagcgg
      301 gtaggaggtg tgcaatcttt ggggggaaca ggtgcacttc gaattggagc tgatttctta
      361 gcgcgttggt acaatggaac aaacaacaag aacacacctg tctatgtgtc ctcaccaacc
      421 tgggagaatc acaatgctgt gttttccgct gctggtttta aagacattcg gtcctatcgc
      481 tactgggatg cagagaagag aggattggac ctccagggct tcctgaatga tctggagaat
      541 gctcctgagt tctccattgt tgtcctccac gcctgtgcac acaacccaac tgggattgac
      601 ccaactccgg agcagtggaa gcagattgct tctgtcatga agcaccggtt tctgtttcca
      661 ttttttgatt cagcctatca gggcttcgca tctggaaacc tggagagaga tgcctgggcc
      721 attcgctatt ttgtgtctga aggcttcgag ttcttctgtg cccagtcctt ctccaagaac
      781 ttcgggctct acaatgagag agtcgggaat ctgactgtgg ttggaaaaga acctgagagc
      841 atcctgcaag tcctttccca gatggagaag atcgtgcgga ttacttggtc caatcccccc
      901 gcccagggag cacgaattgt ggccagcacc ctctctaacc ctgagctctt tgaggaatgg
      961 acaggtaatg tgaagacaat ggctgaccgg attctgacca tgagatctga actcagggca
     1021 cgactagaag ccctcaaaac ccctgggacc tggaaccaca tcactgatca aattggcatg
     1081 ttcagcttca ctgggttgaa ccccaagcag gttgagtatc tggtcaatga aaagcacatc
     1141 tacctgctgc caagtggtcg aatcaacgtg agtggcttaa ccaccaaaaa tctagattac
     1201 gtggccacct ccatccatga agcagtcacc aaaatccagt ga
//
