LOCUS       sequence-133641-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sleeping Beauty (SB) transposon with G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Chicaybam L, Barcelos C, Peixoto B, Carneiro M, Limia CG, Redondo P,
            Lira C, Paraguassu-Braga F, Vasconcelos ZF, Barros L, Bonamino MH
  TITLE     An Efficient Electroporation Protocol for the Genetic Modification 
            of Mammalian Cells.
  JOURNAL   Front Bioeng Biotechnol. 2017 Jan 23;4:99. doi: 
            10.3389/fbioe.2016.00099. eCollection 2016.
  PUBMED    28168187
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2017-01-23"; volume: "4:99. doi"; pages:
            " 10.3389/fbioe.2016.00099. eCollection 2016"
COMMENT     Sequence Label: pT3-Neo
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    425..649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     638..655
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        675..693
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     675..692
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcttgcatgc ctgcaggtcg actctagacc tatacagttg aagtcggaag tttacataca
       61 cttaagttgg agtcattaaa actcgttttt caactactcc acaaatttct tgttaacaaa
      121 caatagtttt ggcaagtcag ttaggacatc tactttgtgc atgacacaag tcatttttcc
      181 aacaattgtt tacagacaga ttatttcact tataattcac tgtatcacaa ttccagtggg
      241 tcagaagttt acatacacta agttgactgt gcctttaaac agcttggaaa attccagaaa
      301 atgatgtcat ggctttagaa gcttctgata gactaattga catcatttga gtcaattgga
      361 ggtgtacctg tggatgtatt tcaaggggat ccattaatgc agctggttct ttccgcctca
      421 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
      481 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
      541 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
      601 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
      661 cagcgagctc tagcatttag gtgacactat agaatagggc cctctagatg catgctcgag
      721 cggccgctta gaaaaa
//
