LOCUS       sequence-133652-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with triple 
            alanine mutations on aminoacids#33,#34 and #37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..71
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtttgctgct ttgctacttc tgcctgctgg ggagcctggg gactttccac accctaactg
       61 acacacattc cacagggtcg acttagatgt agcggtaggt gtcgatgtag cggtaggtgt
      121 cgatgtagcg gtaggtgtcg gccagggcca gggcgcacca ggaatccggt cgcacggagg
      181 acgtgacttc ggccggcgag gcgagactga cgcctatgta gagcccatct tgacccgaat
      241 acttgagctc gctgttctcg ggattggtgc tctctcccgc accagtcatc accggggcac
      301 ccgcccctga ggctgtaggt tgtgttgtgg gggaagtatt tttgctgcaa tcatctgagt
      361 tagaagaggt ggaagttgga gtcatgggaa tggaattatt gctattacca gagcaagtct
      421 ttgatttatt tatgttctta ggttttcgtt tcctggtttg aattccctct tttttcatag
      481 caagtggtct gggcacccca tggagtttca tgtagagtcc acaagcattg cacacgggtt
      541 caccctcggc gtttctgcac cataaggtgg tagttgtggt gtgacatttg gcacaggac
//
