LOCUS       sequence-133657-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with a single
            serine#37 to alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-S37A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..79
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 taaagaatgt cagccttttg catacttctg cctgctgggg agcctgggga ctttccacac
       61 cctaactgac acacattcca agggtcgact tagatgtagc ggtaggtgtc gatgtagcgg
      121 taggtgtcga tgtagcggta ggtgtcggcc agggccaggg cgcaccagga atccggtcgc
      181 acggaggacg tgacttcggc cggcgaggcg agactgacgc ctatgtagag cccatcttga
      241 cccgaatact tgagctcgct gttctcggga ttggtgctct ctcccgcacc agtcatcacc
      301 ggggcacccg cccctgaggc tgtaggttgt gttgtggggg aagtattttt gctgcaatca
      361 tctgagttag aagaggtgga agttggagtc atgggaatgg aattattgct attaccagag
      421 caagtctttg atttatttat gttcttaggt tttcgtttcc tggtttgaat tccctctttt
      481 ttcatagcaa gtggtctggg caccccatgg agtttcatgt agagtccaca agcattgcac
      541 acgggttcac cctcggcgtt tctgcgccat aaggtggtag ttgtggtgtg acagttggca
      601 caggacaatc caagccgccg tgatgaaggc acggcttctg cggcttgatg aggggccggc
      661 tgaggcccgt ttcatcttgc tgtagagccc gcaggcgttg cacaggtaag tgggccggtg
      721 ccgtccccgc cgccacagcg gccgtctgga tggaacccgc agttcacgca ctcgccggct
      781 cttcggacaa ggtcctccag caggtcttgc actgggtact ccgggggcac ccgggaagcg
      841 ggggctcccg gcgcggcttc ttgcaaggct gtgcaccaac cgggggtctt gaaatgggtc
      901 ccggccgggg gccaggcaag gtgttcatgg gcggccccca cagtaacggg ccgagttaag
      961 cggcttctgg actggtgcgt tgttggtt
//
