LOCUS       sequence-133658-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with a single
            Threonine#34 to alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-T34A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..50
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     gap             834..846
                     /estimated_length=13
ORIGIN
        1 gcctgctggg gagcctgggg nctttccaca ccctaactga cacacattcc acnnnnncna
       61 cttagatgta gcggtaggtg tcnatgtagc ggtaggtgtc gatgtagcgg taggtgtcgg
      121 ccagggccag ggcgcaccan gaatccggtc gcacggngga cgtgacttcg gccggcgagg
      181 cnagactgac gcctatgtan agcccatctt gacccgaata cttgagctcg ctgttctcgg
      241 gattggtgct ctctcccgca ccagtcatca ccggggcacc cgccccngag gctgtangtt
      301 gtgttgtggg ggaagtattt ttgctgcaat catctgagtt agaanaggtg gaagttggag
      361 tcatgggaat ggaattattg ctattaccag agcaagtctt tgatttattt atgttcttag
      421 gttttcgttt cctggtttga attccctctt ttttcatanc aagtggtctg ggnancccat
      481 ggagtttcnt gtanagtcna cnagcatngc acannggttc accctcggcg tttctgcncc
      541 ntaangnggn anttgtggtg tgannnttgg cannngacan nnnaagccgc cgtgatgaan
      601 ncacgcgctt ctgcgncttg atgaggggcc ngntgaggnc nttnatcttn ctgtanagcc
      661 cncnngcntt tncacangta nngnccnnnn ccntccnnnc cnccannnng ncntctggan
      721 nnnncnnnca gntcangnnn tcgnngntct nnnnacnngt nnnnnnnnca ntctgnncnn
      781 nnanncnngg ggcnacnngn annnnnnntc cngcnnnnnc tnttnnangn tgtnnnnnnn
      841 nnnnnntntc gnngngnnnn nnnnngggnc na
//
