LOCUS       sequence-133659-        1176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with a single
            Threonine#34 to alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-T34A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..1176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tncnggcgga tcatggacta caaggatgac gatgacaagg actacaagga tgacgatgac
       61 aaggactaca aggatgacga tgacaagatg gccttgactg acggcggctg gtgcttgccg
      121 aagcgcttcg gggccgcggg tgcggacgcc agcgactcca gagcctttcc agcgcgggag
      181 ccctccgcgc cgccttcccc catctcttcc tcgtcctcct cctgctcccg gggcggagag
      241 cggggccccg gcggcgccag caactgcggg acgcctcagc tcgacacgga ggcggcggcc
      301 ggacccccgg cccgctcgct gctgctcagt tcctacgctt cgcatccctt cggggctccc
      361 cacggacctt cggcgcctgg ggtcgcgggc cccgggggca acctgtcgag ctgggaggac
      421 ttgctgctgt tcactgacct cgaccaagcc gcgaccgcca gcaagctgct gtggtccagc
      481 cgcggcgcca agctgagccc cttcgcaccc gagcagccgg aggagatgta ccagaccctc
      541 gccgctctct ccagccaggg tccggccgcc tacgacggcg cgcccggcgg cttcgtgcac
      601 tctgcggccg cggcggcagc agccgcggcg gcggccagct ccccggtcta cgtgcccacc
      661 acccgcgtgg gttccatgct gcccggccta ccgtaccacc tgcaggggtc gggcagtggg
      721 ccagccaacc acgcgggcgg cgcgggcgcg caccccggct ggcctcaggc ctcggccgac
      781 agccctccat acggcagcgg aggcggcgcg gctggcggcg gggccgcggg gcctggcggc
      841 gctggctcag ccgcggcgca cgtctcggcg cgcttcccct actctcccag cccgcccatg
      901 gccaacggcg ccgcgcggga gccgggaggc tacgcgcgcg gcagtggggg cgcgggagcg
      961 tgagcgcgcg cagtagctgc gccatgggcg gcgcgagccc agtacagctc gctgtcgccg
     1021 cgcggcgctg acgggacgta cacaacacaa cacacacaca acattcgagc cctactcgcc
     1081 tacngggggc ccctgagcct gctgncgcgg acctcgaacc ggggctnnag cctgnnancn
     1141 gcggacccgc tccgggccgg ntccatgcaa ctcttg
//
