LOCUS       sequence-133670-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human GATA6 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: tet-pLKO shGATA6 puro
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        118..212
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     129..148
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    194..212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    313..430
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        479..962
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     908..926
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
       61 cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc ggccgcaata
      121 tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaaatccct atcagtgata
      181 gagacttata agttccctat cagtgataga gacaccggcc cagaccactt gctatgaaac
      241 tgcagtttca tagcaagtgg tctgggtttt tgaattctcg acctcgagac aaatggcagt
      301 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      361 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      421 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg
      481 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
      541 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
      601 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
      661 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
      721 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
      781 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
      841 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
      901 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
      961 ttgaccgaat c
//
