LOCUS       sequence-133680-         566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an N-terminally triple FLAG-tagged and C-terminally triple
            AU1-tagged full length human wtGATA6 in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-wtGATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..49
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             50..97
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 gaatttccgg gcccggaatc catggactaa caaggatgac gatgacaagg actacaagga
       61 tgacgatgac aaggactaca aggatgacga tgacaagatg gccttgactg acggcggctg
      121 gtgcttgccg aagcgcttcg gggccgcggg tgcggacgcc agcgactcca gagcctttcc
      181 agcgcgggag ccctccacgc cgccttcccc catctcttcc tcgtcctcct cctgctcccg
      241 gggcggagag cggggccccg gcggcgccag caactgcggg acgcctcagc tcgacacgga
      301 ggcggcggcc ggacccccgg cccgctcgct gctgctcagt tcctacgctt cgcatccctt
      361 cggggctccc cacggacctt cggcgcctgg ggtcgcgggc cccgggggca acctgtcgag
      421 ctgggaggac ttgctgctgt tcactgacct ctaccaagcc gcgaccgcca gcaagctgct
      481 gtggtccagc cgcggcgcca agctgagccc cttcgcaccc gagcagccgg aggagatgta
      541 ccataccctc gccgctctca ccatcc
//
