LOCUS       sequence-133681-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an N-terminally triple FLAG-tagged and C-terminally triple
            AU1-tagged full length human wtGATA6 in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-wtGATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..75
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(930..959)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gaagtaatct gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
       61 actgacacac attccacagg gtcgacttag atgtagcggt aggtgtcgat gtagcggtag
      121 gtgtcgatgt agcggtaggt gtcggccagg gccagggcgc accaggaatc cggtcgcacg
      181 gaggacgtga cttcggccgg cgaggcgaga ctgacgccta tgtagagccc atcttgaccc
      241 gaatacttga gctcgctgtt ctcgggattg gtgctctctc ccgcaccagt catcaccggg
      301 gcacccgccc ctgaggctgt aggttgtgtt gtgggggaag tatttttgct gcaatcatct
      361 gagttagaag aggtggaagt tggagtcatg ggaatggaat tattgctatt accagagcaa
      421 gtctttgatt tatttatgtt cttaggtttt cgtttcctgg tttgaattcc ctcttttttc
      481 atagcaagtg gtctgggcac cccatggagt ttcatgtaga gtccacaagc attgcacacg
      541 ggttcaccct cggcgtttct gcgccataag gtggtagttg tggtgtgaca gttggcacag
      601 gacaatccaa gccgccgtga tgaaggcacg cgcttctgcg gcttgatgag gggccggctg
      661 aggccgttca tcttgctgta gagcccgcag gcgttgcaca ggtagtggcc ggtgccgtcc
      721 cgccgccaca gcggcgtctg gatggagccg cagttcacgc actcgcggct ctcggacagg
      781 tcctccagca ggtctgcact gggaccccgg ggcaccggga gcggggctcc ggcgcggctc
      841 tgcaggctgt gcagcaccgg ggtctcgaag ggtccggcgg gccaggcagg cgtcagtggc
      901 gcccccacgt agggcgagta ggggctcgga tggtggtggt ggtggtggtg gtggtggtgg
      961 tacgtcccgt tcagcggccg ccgcggccga cagcgagctg
//
