LOCUS       sequence-133684-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dox inducible knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Kim D, Fiske BP, Birsoy K, Freinkman E, Kami K, Possemato RL, 
            Chudnovsky Y, Pacold ME, Chen WW, Cantor JR, Shelton LM, Gui DY, 
            Kwon M, Ramkissoon SH, Ligon KL, Kang SW, Snuderl M, Vander Heiden 
            MG, Sabatini DM
  TITLE     SHMT2 drives glioma cell survival in ischaemia but imposes a 
            dependence on glycine clearance.
  JOURNAL   Nature. 2015 Apr 16;520(7547):363-7. doi: 10.1038/nature14363. Epub 
            2015 Apr 8.
  PUBMED    25855294
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14363"; journalName: "Nature"; date: "2015-04-16- 
            16"; volume: "520"; issue: "7547"; pages: "363-7"
COMMENT     Sequence Label: shGLDC-DOX
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    115..232
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(333..427)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    333..351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    361..379
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(397..416)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(716..760)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc
       61 aaccccctcg agccgcggcc aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt
      121 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
      181 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
      241 gaatactgcc atttgtctcg aggtcgagaa ttcaaaaaga agtttatgag tctccatttc
      301 tcgagaaatg gagactcata aacttcccgg tgtctctatc actgataggg aacttataag
      361 tctctatcac tgatagggat ttcacgttta tggtgatttc ccagaacaca tagcgacatg
      421 caaatattgc ggccgctcga ggctagtctc gtgatcgata ccgtcgagat ccgttcacta
      481 atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt
      541 cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa
      601 ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta
      661 tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac
      721 ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt
      781 cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc
      841 aggtcgtgtg attccaaatc tgttccagan atttattact ccaactagca ttccaaggca
      901 cagcagtggt gcaantgagt tttccagagc aaccccaaat ccccangagc tgttgatcct
      961 ttaggtatct ttccacagcc aggat
//
