LOCUS       sequence-133687-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Hara T, Takamura A, Kishi C, Iemura S, Natsume T, Guan JL, Mizushima
            N
  TITLE     FIP200, a ULK-interacting protein, is required for autophagosome 
            formation in mammalian cells.
  JOURNAL   J Cell Biol. 2008 May 5. 181(3):497-510.
  PUBMED    18443221
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2008 May 5. 181(3):497-510."
COMMENT     Sequence Label: pMXs-IP-EGFP-hFIP200
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     310..326
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      458..467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(464..466,467..469,470..982)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(509..530)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(770..789)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatgagatct tatatggggc acccccgccc cttgtaaact tccctgaccc tgacatgaca
       61 agagttacta acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac
      121 gaagtctgga gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct
      181 cacccttacc gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa
      241 cctcgctgga aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac
      301 ggcatcgcag cttggataca cgccgcccac gtgaaggctg ccgaccccgg gggtggacca
      361 tcctctagac tgccggatct agctagttaa ttaaggatcc cagtgtggtg gtacgggaat
      421 tcctgcaggc ctcgagggcc ggcgcgccgc ggccgccgcc accatggtga gcaagggcga
      481 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      541 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      601 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      661 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      721 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      781 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      841 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      901 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      961 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1021 cacccccatc ggcgacggcc ccgtgctgct gccc
//
