LOCUS       sequence-133694-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam 
            JW, Constantine CZ, Kappock TJ
  TITLE     Structure of a NADH-insensitive hexameric citrate synthase that 
            resists acid inactivation.
  JOURNAL   Biochemistry. 2006 Nov 14;45(45):13487-99.
  PUBMED    17087502
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-11-14"; volume: "45(45)"; pages: 
            "13487-99"
COMMENT     Sequence Label: pJK136
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gagcgcgtcg cagaaagaag
       61 gtaagctatc taccgctacc atttcggttg atggaaaatc cgccgaaatg cctgtgcttt
      121 caggcactct gggaccggat gttatcgaca tccgcaaact tccggcgcaa ctgggcgttt
      181 tcacgttcga cccaggttac ggggaaacag cggcctgcaa cagcaaaatc acctttattg
      241 atggtgataa aggcgttctg ctgcaccgtg gttaccctat tgcgcagctg gccgaaaatg
      301 cttcctacga agaagttatt tatctgcttt tgaatggcga actgcccaac aaggcgcagt
      361 acgacacctt caccaacacc cttacaaacc atacgctgct gcacgagcag atccgtaact
      421 tctttaacgg cttccggcgt gatgcccacc caatggccat tctgtgtggt acggttgggg
      481 ctttgtctgc cttctaccca gatgccaacg atattgccat tcccgccaat cgggatctgg
      541 ccgccatgcg gctgattgcc aaaatcccaa ccattgcggc atgggcttac aaatacacgc
      601 agggtgaagc ctttatctac ccgcggaatg atctgaacta cgcagaaaac ttcctgtcca
      661 tgatgttcgc gcgcatgtcc gaaccttaca aggtcaaccc tgttctggcc cgcgccatga
      721 accggattct gattctgcat gccgatcatg agcagaatgc ctctacctcc accgtacgtc
      781 tggctggttc tacaggggcc aatccgtttg cctgtattgc tgcgggcatt gccgctctgt
      841 ggggacctgc acatggtggc gcaaacgaag ctgtgctgaa aatgctggcc cgtattggca
      901 agaaagaaaa tattcctgcc tttatcgcac aggtgaagga caagaacagc ggcgtaaagc
      961 tgatgggctt tggccaccgc gtttacaaga acttcgaccc acgtgcgaag atcatgcagc
     1021 agacctgcca cgaagtgctg acagaacttg gca
//
