LOCUS       sequence-133695-        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam 
            JW, Constantine CZ, Kappock TJ
  TITLE     Structure of a NADH-insensitive hexameric citrate synthase that 
            resists acid inactivation.
  JOURNAL   Biochemistry. 2006 Nov 14;45(45):13487-99.
  PUBMED    17087502
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-11-14"; volume: "45(45)"; pages: 
            "13487-99"
COMMENT     Sequence Label: pJK136
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcttagcgtt tggcaagcgg cacatagtca
      121 cgctgcggtg cgccaatata aagctggcga gggcggctga tacgctggcc cggttcttca
      181 atcatttcct tccactggct cacccagccg gtggtgcggg ctacggcaaa cagcacagta
      241 aacatactgg tggggatgcc catggccttg agaatgatgc cagagtagaa atccacattc
      301 gggtaaagtt tgcgctgcac gaagtaatca tcgctcagag caatcttttc cagctcaacc
      361 gccagatcca gcagcggatc atccttaatg ccaagttctg tcagcacttc gtggcaggtc
      421 tgctgcatga tcttcgcacg tgggtcgaag ttcttgtaaa cgcggtggcc aaagcccatc
      481 agctttacgc cgctgttctt gtccttcacc tgtgcgataa aggcaggaat attttctttc
      541 ttgccaatac gggccagcat tttcagcaca gcttcgtttg cgccaccatg tgcaggtccc
      601 cacagagcgg caatgcccgc agcaatacag gcaaacggat tggcccctgt agaaccagcc
      661 agacgtacgg tggaggtaga ggcattctgc tcatgatcgg catgcagaat cagaatccgg
      721 ttcatggcgc gggccagaac agggttgacc ttgtaaggtt cggacatgcg cgcgaacatc
      781 atggacagga agttttctgc gtagttcaga tcattccgcg ggtagataaa ggcttcaccc
      841 tgcgtgtatt tgtaagccca tgccgcaatg gttgggattt tggcaatcag ccgcatggcg
      901 gccagatccc gattggcggg aatggcaata tcgttggcat ctgggtagaa ggcagacaaa
      961 gccccaaccg taccacacag aatggccatt gggtgggcat cacgccggaa gccgttaaag
     1021 aagttacgga tctgctcgtg cagcagcgta tggtttgtaa gggtgttggt gaaggtgtcg
     1081 tactgcgcct tgttgggcag ttcgccattc aaaagcagat aaataacttc ttcgtaggaa
     1141 gca
//
