LOCUS       sequence-133697-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E.
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam 
            JW, Constantine CZ, Kappock TJ
  TITLE     Structure of a NADH-insensitive hexameric citrate synthase that 
            resists acid inactivation.
  JOURNAL   Biochemistry. 2006 Nov 14;45(45):13487-99.
  PUBMED    17087502
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-11-14"; volume: "45(45)"; pages: 
            "13487-99"
COMMENT     Sequence Label: pJK225
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgagc gcgtcgcaga aagaaggtaa
       61 gctatctacc gctaccattt cggttgatgg aaaatccgcc gaaatgcctg tgctttcagg
      121 cactctggga ccggatgtta tcgacatccg caaacttccg gcgcaactgg gcgttttcac
      181 gttcgaccca ggttacgggg aaacagcggc ctgcaacagc aaaatcacct ttattgatgg
      241 tgataaaggc gttctgctgc accgtggtta ccctattgcg cagctggccg aaaatgcttc
      301 ctacgaagaa gttatttatc tgcttttgaa tggcgaactg cccaacaagg cgcagtacga
      361 caccttcacc aacaccctta caaaccatac gctgctgcac gagcagatcc gtaacttctt
      421 taacggcttc cggcgtgatg cccacccaat ggccattctg tgtggtacgg ttggggcttt
      481 gtctgccttc tacccagatg ccaacgatat tgccattccc gccaatcggg atctggccgc
      541 catgcggctg attgccaaaa tcccaaccat tgcggcatgg gcttacaaat acacgcaggg
      601 tgaagccttt atctacccgc ggaatgatct gaactacgca gaaaacttcc tgtccatgat
      661 gttcgcgcgc atgtccgaac cttacaaggt caaccctgtt ctggcccgcg ccatgaaccg
      721 gattctgatt ctgcatgccg atcatgagca gaatgcctct acctccaccg tacgtctggc
      781 tggttctaca ggggccaatc cgtttgcctg tattgctgcg ggcattgccg ctctgtgggg
      841 acctgcacat ggtggcgcaa acgaagctgt gctgaaaatg ctggcccgta ttggcaagaa
      901 agaaaatatt cctgccttta tcgcacaggt gaaggacaag aacagcggcg taaagctgat
      961 gggctttggc caccgcgttt acaagaactt cgacccacgt gcgaagatca tgcagcagac
     1021 ctgccacgaa gtgctgacag aacttggcat t
//
