LOCUS       sequence-133700-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YAP/TAZ-responsive synthetic promoter driving luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, 
            Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, 
            Piccolo S
  TITLE     Role of YAP/TAZ in mechanotransduction.
  JOURNAL   Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137.
  PUBMED    21654799
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-8- 8"; volume: "474"; issue: "7350"; pages:
            "179-83"
COMMENT     Sequence Label: 8xGTIIC-luciferase
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(73..79,80..81,82..88)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(105..111,112..113,114..120)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(159..165,166..167,168..174)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(191..197,198..199,200..206)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     primer_bind     232..248
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     233..249
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(456..474)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gccagaacat ttctctatcg ataggtaccg agctcttacg cgtgctagcc cggccagtgc
       61 caagttgaga cacattccac acattccact gcaagcttga gacacattcc acacattcca
      121 ctgcaagctt ctagcccggc cagtgccaag ttgagacaca ttccacacat tccactgcaa
      181 gcttgagaca cattccacac attccactgc aagcttctag agatctgcag gtcgaggtcg
      241 acggtatcga taagcttggg ggtgggcgcc ggggggacct taaagcctct gccccccaag
      301 gagcccttcc cagacagccg ccggcaccca ccgctccgtg ggacgatccc ccaagagctt
      361 ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata aagaaaggcc
      421 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
      481 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
      541 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
      601 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
      661 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
      721 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
      781 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
      841 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
      901 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
      961 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
     1021 ctcatagaac tgcctgcgtg agattctcgc atgccagag
//
