LOCUS       sequence-133706-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam 
            JW, Constantine CZ, Kappock TJ
  TITLE     Structure of a NADH-insensitive hexameric citrate synthase that 
            resists acid inactivation.
  JOURNAL   Biochemistry. 2006 Nov 14;45(45):13487-99.
  PUBMED    17087502
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-11-14"; volume: "45(45)"; pages: 
            "13487-99"
COMMENT     Sequence Label: pJK227
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatgagcgc gtcgcagaaa
       61 gaaggtaagc tatctaccgc taccatttcg gttgatggaa aatccgccga aatgcctgtg
      121 ctttcaggca ctctgggacc ggatgttatc gacatccgca aacttccggc gcaactgggc
      181 gttttcacgt tcgacccagg ttacggggaa acagcggcct gcaacagcaa aatcaccttt
      241 attgatggtg ataaaggcgt tctgctgcac cgtggttacc ctattgcgca gctggccgaa
      301 aatgcttcct acgaagaagt tatttatctg cttttgaatg gcgaactgcc caacaaggcg
      361 cagtacgaca ccttcaccaa cacccttaca aaccatacgc tgctgcacga gcagatccgt
      421 aacttcttta acggcttccg gcgtgatgcc cacccaatgg ccattctgtg tggtacggtt
      481 ggggctttgt ctgccttcta cccagatgcc aacgatattg ccattcccgc caatcgggat
      541 ctggccgcca tgcggctgat tgccaaaatc ccaaccattg cggcatgggc ttacaaatac
      601 acgcagggtg aagcctttat ctacccgcgg aatgatctga actacgcaga aaacttcctg
      661 tccatgatgt tcgcgcgcat gtccgaacct tacaaggtca accctgttct ggcccgcgcc
      721 atgaaccgga ttctgattct gcatgccgat catgagcaga atgcctctac ctccaccgta
      781 cgtctggctg gttctacagg ggccaatccg tttgcctgta ttgctgcggg cattgccgct
      841 ctgtggggac ctgcacatgg tggcgcaaac gaagctgtgc tgaaaatgct ggcccgtatt
      901 ggcaagaaag aaaatattcc tgcctttatc gcacaggtga aggacaagaa cagcggcgta
      961 aagctgatgg gctttggcca ccgcgtttac aagaacttcg acccacgtgc gaagatcatg
     1021 cagcagacct gccacgaagt gctga
//
