LOCUS       sequence-133725-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK280
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             627..644
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(697..715)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      711..758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(882..901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgatg agcgaaaccg cccccctgcc
       61 ctctgcttcc agtgctttag aagataaagc agcctctgcc cccgttgtgg gcattatcat
      121 gggcagccag tcagactggg aaaccatgcg ccatgcagac gcattgctga cagagctgga
      181 aattccgcat gaaaccctca ttgtttcagc acatcgtacg ccagaccggt tggcggatta
      241 tgcgcgtacg gcagccgaac gcggcctgaa tgtgattatt gctggggccg gtggagctgc
      301 atttctgccg ggtatgtgtg cggcatggac acgcctgccc gtgctgggtg ttccagtaga
      361 atctcgcgct cttaaaggca tggacagcct gctttccatt gtgcagatgc ccggcggcgt
      421 gcctgtgggc actttggcta ttggggcatc cggcgccaaa aatgccgcct tgctggccgc
      481 ctccattctt gcactgtata acccggccct tgccgcacgc cttgaaacat ggcgcgcatt
      541 gcagacggcc tctgtcccca actctcccat taccgaagat aaatgagaat tcgagctccg
      601 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      661 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      721 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      781 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      841 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      901 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      961 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     1021 ttcacgta
//
