LOCUS       sequence-133727-        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  specific sgRNA against mouse Dicer1 gene cloned in the pX330 
            backbone (Addgene Number 42230). Generation of Dicer1 knockout 
            mESCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Bodak M, Cirera-Salinas D, Yu J, Ngondo RP, Ciaudo C
  TITLE     Dicer, a new regulator of pluripotency exit and LINE-1 elements in 
            mouse embryonic stem cells.
  JOURNAL   FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 
            10.1002/2211-5463.12174. eCollection 2017 Feb.
  PUBMED    28174687
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/2211-5463.12174"; journalName: "FEBS Open Bio"; date: 
            "2017-01-11- 11"; volume: "7"; issue: "2"; pages: "204-220"
COMMENT     Sequence Label: pX330-sgRNA_Dicer_1
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(236..311)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(333..581)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(391..410)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(561..581)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(723..742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
       61 tggcgttact attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
      121 taccgtaagt tatgtaacgg gtacctctag agccatttgt ctgcagaatt ggcgcacgcg
      181 ctaaaaacgg actagcctta ttttaacttg ctatttctag ctctaaaaca aaaaagcacc
      241 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatttct
      301 agctctaaaa ccgcctgtag gtttaaactc ccggtgtttc gtcctttcca caagatatat
      361 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
      421 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
      481 actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat
      541 cgtatatgca aatatgaagg aatcatggga aataggccct cacatgtgag caaaaggcca
      601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aa
//
