LOCUS       sequence-133729-        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with triple 
            alanine mutations on aminoacids#33,#34 and #37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..75
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             1045..1074
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 atggactaca aggatgacga tgacaaggac tacaaggatg acgatgacaa ggactacaag
       61 gatgacgatg acaagatggc cttgactgac ggcggctggt gcttgccgaa gcgcttcggg
      121 gccgcgggtg cggacgccag cgactccaga gcctttccag cgcgggagcc cgccgcgccg
      181 cctgccccca tctcttcctc gtcctcctcc tgctcccggg gcggagagcg gggccccggc
      241 ggcgccagca actgcgggac gcctcagctc gacacggagg cggcggccgg acccccggcc
      301 cgctcgctgc tgctcagttc ctacgcttcg catcccttcg gggctcccca cggaccttcg
      361 gcgcctgggg tcgcgggccc cgggggcaac ctgtcgagct gggaggactt gctgctgttc
      421 actgacctcg accaagccgc gaccgccagc aagctgctgt ggtccagccg cggcgccaag
      481 ctgagcccct tcgcacccga gcagccggag gagatgtacc agaccctcgc cgctctctcc
      541 agccagggtc cggccgccta cgacggcgcg cccggcggct tcgtgcactc tgcggccgcg
      601 gcggcagcag ccgcggcggc ggccagctcc ccggtctacg tgcccaccac ccgcgtgggt
      661 tccatgctgc ccggcctacc gtaccacctg caggggtcgg gcagtgggcc agccaaccac
      721 gcgggcggcg cgggcgcgca ccccggctgg cctcaggcct cggccgacag ccctccatac
      781 ggcagcggag gcggcgcggc tggcggcggg gccgcggggc ctggcggcgc tggctcagcc
      841 gcggcgcacg tctcggcgcg cttcccctac tctcccagcc cgcccatggc caacggcgcc
      901 gcgcgggagc cgggaggcta cgcggcggcg ggcagtgggg gcgcgggagg cgtgagcggc
      961 ggcggcagta gcctggcggc catgggcggc cgcgagcccc agtacagctc gctgtcggcc
     1021 gcgcggccgc tgaacgggac gtaccaccac caccaccacc accaccacca ccatccgagc
     1081 ccctactcgc cctacgtggg ggcgccactg acgcctgcct ggcccgccgg acccttcgag
     1141 accccggtgc tgcacagcct gcagagccgc gccggagccc cgctcccggt gccccggggt
     1201 cccagtgcag acctgctgga ggacctgtcc gagagccgcg agtgcgtgaa ctgcggctcc
     1261 atccagacgc cgctgtggcg gcgggacggc accggccact acctgtgcaa cgcctgcggg
     1321 ctctacagca agatgaacgg cctcagccgg cccctcatca agccgcagaa gcgcgtgcct
     1381 tcatcacggc ggcttggatt gtcctgtgcc aactgtcaca ccacaactac caccttatgg
     1441 cgcagaaacg ccgagggtga acccgtgtgc aatgcttgtg gactctacat gaaactccat
     1501 ggggtgccca gaccacttgc tatgaaaaaa gagggaattc aaaccaggaa acgaaaacct
     1561 aagaacataa ataaatcaaa gacttgctct ggtaatagca ataattccat tcccatgact
     1621 ccaacttcca cctcttctaa ctcagatgat tgcagcaaaa atacttcccc cacaacacaa
     1681 cctacagcct caggggcggg tgccccggtg atgactggtg cgggagagag caccaatccc
     1741 gagaacagcg agctcaagta ttcgggtcaa gatgggctct acataggcgt cagtctcgcc
     1801 tcgccggccg aagtcacgtc ctccgtgcga ccggattcct ggtgcgccct ggccctggcc
     1861 gacacctacc gctacatcga cacctaccgc tacatcgaca cctaccgcta catctaa
//
