LOCUS       sequence-133732-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Fujita T, Fujii H
  TITLE     Efficient isolation of specific genomic regions and identification 
            of associated proteins by engineered DNA-binding molecule-mediated 
            chromatin immunoprecipitation (enChIP) using CRISPR.
  JOURNAL   Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 
            10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
  PUBMED    23942116
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2013.08.013"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-09-13- 13"; volume: "439"; issue: "1"; pages: 
            "132-6"
COMMENT     Sequence Label: 3xFLAG-dCas9/pCMV-7.1
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gctcatggac
      121 aagaagtact ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg
      181 gacgagtaca aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc
      241 ataaagaaga acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg
      301 cggctcaaaa gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg
      361 caggagatct ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag
      421 gagtcctttt tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc
      481 gtggacgagg tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt
      541 gtagacagta ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc
      601 aaatttcggg gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac
      661 aaactcttta tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac
      721 gcatccggag ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc
      781 gaaaacctca tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc
      841 gccctgtcac tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc
      901 aagcttcaac tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc
      961 ggcgaccagt acgcagacct tt
//
