LOCUS       sequence-133733-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Antisense control vector for functional testing (ColE1 ori, AmpR, 
            strong promoter/terminator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-anti-control
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      245..833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     734..753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     987..1004
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
       61 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      121 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      181 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      601 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      661 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      721 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      781 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca
      841 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      901 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      961 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcagtctag
     1021 actcgagtaa ggatccaaaa aa
//
