LOCUS       sequence-133735-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Antisense control vector for functional testing (ColE1 ori, AmpR, 
            strong promoter/terminator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-anti-control
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(206..310)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     378..396
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(400..443)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        461..495
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(600..617)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(851..870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
       61 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
      121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
      181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
      241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
      301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
      361 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
      421 tagctttcgc taaggatgat ttctggaatt ctaaagatct ttgacagcta gctcagtcct
      481 aggtataata ctagtgcctc tacctgcttc ggccgataaa gccgacgata atactcccaa
      541 agcccgccga aaggcgggct tttttttgga tccttactcg agtctagact gcaggcttcc
      601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
      781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1021 tgtgtgcacg aaccccc
//
