LOCUS       sequence-133737-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., 
            Nat. Chem. Biol., 2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAOUTA4
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      246..834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     735..754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     988..1005
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
       61 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      121 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      181 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
      241 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      301 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      361 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      421 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      481 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      541 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      601 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctgtg gaaaaacgcc
      841 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      901 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      961 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ctgcagtcta
     1021 gactcgagta aggatccaaa aaa
//
