LOCUS       sequence-133744-        2169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse Drp1 isoform b (699amino acids) with 
            C-terminal His-tag and PreScission protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Liu R, Chan DC
  TITLE     The mitochondrial fission receptor Mff selectively recruits 
            oligomerized Drp1.
  JOURNAL   Mol Biol Cell. 2015 Dec 1;26(24):4466-77. doi: 
            10.1091/mbc.E15-08-0591. Epub 2015 Oct 7.
  PUBMED    26446846
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-08-0591"; journalName: "Mol Biol Cell"; date: 
            "2015-12-1- 1"; volume: "26"; issue: "24"; pages: "4466-77"
COMMENT     Sequence Label: pET21-Drp1
FEATURES             Location/Qualifiers
     source          1..2169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2107..2130
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2152..2169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaggcgc tgatcccggt catcaataag ctgcaggacg tcttcaacac agtgggtgcg
       61 gacatcatcc agctgcctca gatcgtcgta gtgggaacgc agagcagtgg gaagagctca
      121 gtgctggaaa gcctagtggg cagggacctt cttcccagag gaactggtgt ggtcacccgg
      181 agacctctca ttctgcagct agtccacgtt tcaccagaag ataaaagaaa aacaacagga
      241 gaagaaaatg gagttgaagc agaagaatgg ggtaaatttc ttcacaccaa aaacaagctt
      301 tacacagatt ttgatgaaat tcgacaagaa attgaaaatg aaactgaaag aatttcagga
      361 aataataagg gggtaagccc tgagccaatc catctcaagg ttttctcgcc caacgttgtc
      421 aacctgacac ttgtggattt accgggaatg accaaagtac ctgtaggcga tcagcccaag
      481 gacatcgagc ttcagatcag agaacttatt cttcggttca tcagtaatcc caattccatt
      541 atcctcgccg tcactgctgc aaatacagat atggcaacat cagaagcact caagatttcc
      601 agagaggtag atccagatgg gcgcagaact ctagctgtaa tcactaaact tgatctcatg
      661 gatgcgggta ctgatgccat ggatgtattg atgggaaggg ttattccagt caagcttgga
      721 ataattggag tagttaacag aagccaactg gatattaaca ataagaagag tgtaactgat
      781 tcaatccgtg atgagtatgc ttttcttcaa aagaagtacc catctctggc caacagaaat
      841 ggaacaaagt atcttgctag gaccctgaat aggttactta tgcatcatat cagagattgt
      901 ttaccagagc tgaaaacaag aataaatgtc ttagctgctc agtatcagtc tcttctaaat
      961 agctatggtg aaccggtgga tgataaaagt gctactttac tccagcttat taccaaattt
     1021 gccacagagt attgtaacac gattgaagga accgcaaagt acattgaaac ttctgagcta
     1081 tgcggtggtg ctaggatttg ttatattttc catgagactt tcgggcgaac cttagaatct
     1141 gtggacccac taggtggcct taacactatt gacatcctga ctgccatcag aaatgctact
     1201 ggcccccgtc ctgctttatt tgtgcctgaa gtttcatttg agttactggt caaacgtcag
     1261 attaagcgtc tagaagagcc cagcctacgg tgtgtggagc tggtccatga ggagatgcag
     1321 aggatcattc agcattgtag caattacagc acacaggaat tgttacggtt ccctaaactt
     1381 cacgatgcca tagttgaagt agtgacctgt cttcttcgta aaaggttgcc cgtgacaaat
     1441 gaaatggtgc ataacttagt ggcaattgag ctagcgtata tcaacacaaa acaccccgac
     1501 tttgctgatg cctgtgggct aatgaacaat aatatagagg aacaaagaag aaacaggcta
     1561 gcaagagagc tgccttcagc tggatcacgg gacaaggtcc catctgcagg tggtgggatt
     1621 ggagacggtg gtcaggaacc aacaacaggc aactggagag gaatgctgaa aacttcaaaa
     1681 gctgaagaat tacttgctga agaaaaatca aaaccaattc caattatgcc agcaagtcca
     1741 cagaaaggcc atgctgtcaa tttgctagat gtgccagttc cagttgcaag aaaactgtct
     1801 gcccgagaac agcgagattg tgaggttatt gaaagactta tcaaatcata ttttctaatt
     1861 gtcagaaaga atattcaaga cagtgtccca aaggcagtaa tgcatttttt ggttaatcat
     1921 gtgaaagata ctcttcagag tgaactggta gggcagctgt ataagtcatc cttattagat
     1981 gaccttctga ctgaatccga ggacatggcc caacgaagaa aagaagcagc ggatatgctg
     2041 aaggcattac aaggagccag tcaaattatt gctgaaatcc gagagactca tctttgggcg
     2101 gatccgctgg aagttctgtt ccaggggccc aagcttgcgg ccgcactcga gcaccaccac
     2161 caccaccac
//
