LOCUS       sequence-133763-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9-VRQR-HF1 variant: 
            CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, 
            G1218R, R1335Q, T1337R)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2443
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgcgaaagc agcggacttt cgacaacggt agcattccac atcaaatcca cttaggcgaa
       61 ttgcatgcta tacttagaag gcaggaggat ttttatccgt tcctcaaaga caatcgtgaa
      121 aagattgaga aaatcctaac ctttcgcata ccttactatg tgggacccct ggcccgaggg
      181 aactctcggt tcgcatggat gacaagaaag tccgaagaaa cgattactcc ctggaatttt
      241 gaggaagttg tcgataaagg tgcgtcagct caatcgttca tcgagaggat gaccgccttt
      301 gacaagaatt taccgaacga aaaagtattg cctaagcaca gtttacttta cgagtatttc
      361 acagtgtaca atgaactcac gaaagttaag tatgtcactg agggcatgcg taaacccgcc
      421 tttctaagcg gagaacagaa gaaagcaata gtagatctgt tattcaagac caaccgcaaa
      481 gtgacagtta agcaattgaa agaggactac tttaagaaaa ttgaatgctt cgattctgtc
      541 gagatctccg gggtagaaga tcgatttaat gcgtcacttg gtacgtatca tgacctccta
      601 aagataatta aagataagga cttcctggat aacgaagaga atgaagatat cttagaagat
      661 atagtgttga ctcttaccct ctttgaagat cgggaaatga ttgaggaaag actaaaaaca
      721 tacgctcacc tgttcgacga taa
//
