LOCUS       sequence-133774-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of The CFP-tagged GIV-F1685A biosensors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     Mammalian expression of The CFP-tagged GIV-WT biosensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CFP-GIV-F1685A
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagact
      121 cgagcggccg catggagaac gaaattttta ctccccttct ggagcagttc atgaccagcc
      181 ctttggtcac ttgggttaaa acgtttggac ctctggccgc aggaaatggg accaaccttg
      241 atgaatatgt ggctttggtg gatggggtat tcttgaacca ggtcatgctc caaattaatc
      301 ctaaattgga gagtcagaga gtaaataaaa aagtcaataa tgatgcctca cttagaatgc
      361 acaatctatc cattttggtg agacagataa aattttatta ccaggagact ttgcagcaat
      421 tgatcatgat gtcgttgcca aatgtcttaa tcattggcaa aaatcccttt tctgaacaag
      481 gcacagaaga agttaaaaaa ctgcttttac ttttattggg ttgtgcagtt cagtgtcaga
      541 aaaaagagga atttattgaa agaattcaag gtttagattt tgatacaaaa gcagcggttg
      601 ccgcacatat tcaagaggta actcataatc aggaaaatgt gtttgacctg caatggatgg
      661 aagtgactga tatgtcgcag gaggacatag aaccactctt gaaaaatatg gcattgcatc
      721 taaaaagact tatagatgag agagatgaac attcagagac tatcatagaa ctctctgaag
      781 agcgggatgg tctccatttt ctaccccatg cctcttcatc tgcacagtca ccctgtgg
//
