LOCUS       sequence-133775-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of The CFP-tagged GIV-F1685A biosensors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     Mammalian expression of The CFP-tagged GIV-WT biosensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CFP-GIV-F1685A
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(16..729,730..732,733..735))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(61..82)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     410..429
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     669..690
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttaagcttg gtaccttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
       61 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      121 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      181 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
      241 gtgggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgatgta
      301 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      361 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
      421 gccgtcgtcc ttgaagaaga tggtacgctc ctggacgtag ccttcgggca tggcggactt
      481 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccccaggt
      541 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
      601 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacctgtg
      661 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
      721 gcccttgctc accatggatc cggcgccgga tcctgaggag ctttgttgct ccctagacct
      781 gctttttgaa tttctgcttt cttgtacttt gtccacatta gatgctgcag tggtgttgct
      841 gtcagcagca gctggtggtg aatcaactga tataggcagt ctactgtcct tggtcaaaaa
      901 atcatggatg cttgtccttc gtgtagttcc ttcggcagtt gagatcacgc tgcttgcacg
      961 aggtaaagtt gcat
//
