LOCUS       sequence-133796-         452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 Destination vector - Position B - Assembly of 4-5 TU's from 
            Level 1-ABCD or ABCDE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-B-lacZ
FEATURES             Location/Qualifiers
     source          1..452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..51
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     40..62
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     59..75
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     59..75
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             84..95
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggtctcagta catctagaga cgtctagaca tatgttgtga gcggataaca atttcacaca
       61 ggaaacagct atgaccatgg tacaaagagg agaaaggaca tgggtgaccc gaaactgctg
      121 ctggaagttc cgcacgctat ctcttcttct ccgggtaact ctctggctgt tgttctgcaa
      181 cgtcgtgact gggaaaaccc gggtgttacc cagctgaacc gtctggctgc tcacccgccg
      241 ttcgcttctt ggcgtaactc tgaagaagct cgtaccgacc gtccgtccca gcagctgcgt
      301 tctctgatcc gtctgctgac caaaccggaa cgtaaactgt cttggctgct gccgccgctg
      361 tctaacaact aacgcaaaaa accccgcccc tgacagggcg gggttttttc gcactagtgg
      421 atccgcatgc cgtctcatta gggacagaga cc
//
