LOCUS       sequence-13380-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-sCS4-2T
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             265..282
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             514..531
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 catatgcacc accaccacca ccacaagaaa ttattaatct ccaaaattcc atttgcaagg
       61 ctagtgaaag aagttacaga cgagtttaca actaaagatc aggatttacg ttggcagtca
      121 atggcgatta tggcgttaca ggaagcaagc gaagcgtatc tggtaggatt attggaacat
      181 acaaacctct tggcgctgca tgcaaaaaga attactataa tgaagaaaga catgcaacta
      241 gcaagaagaa tcaggggaca gtttctggtt ccgcgtggat ccatggagcg ccacaaacta
      301 gcagacgaaa acatgagaaa agtgtggtcc aacatcataa gcaaatacga gtctattgaa
      361 gaacaaggag acctcgtaga cctgaaaact ggtgaaatag ttgaagataa tgggcatatc
      421 aagacgctga cggccaacaa cagcacgaaa gataaaagaa caaaatacac aagcgtgctc
      481 agagacatta tcgacatcag cgacgaggaa gatctggttc cgcgtggatc caagcgtatt
      541 tctggtttga tctacgaaga agtcagagcc gtcttgaaat ccttcttgga atccgtcatc
      601 agggactctg ttacttacac tgaacacgcc aagagaaaga ctgttacttc tttggatgtt
      661 gtttatgctt tgaagagaca aggtagaacc ttatatggtt tcggtggtta actcgag
//
